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来自默克密理博美国总部的资深流式应用科学家Brain Eric Hall博士,在新流式 新思维” 2015默克密理博生物科学亚洲峰会中分享了成像流式的最新进展,参会者反应热烈,提问踊跃。为了让更多的未参会者体验成像流式技术的魅力,现将该讲座公布如下。


演讲者Dr Brian Eric Hall, Senior Application Scientist, Merck Millipore
演讲主题Protein Aggregation and Applications using the ImageStreamHigh Content Analyzer
主题摘要
The ImageStream is a multispectral imaging flow cytometer that generates high resolution images of cells at a rate of 1000's of cells per second. This allows for the rapid acquisition of tens of thousands of images per sample. Using the IDEAS image analysis software, the system calculates features based not only on fluorescence intensity but the morphology of that fluorescence as well. With this novel approach the ImageStream is able to seamlessly combine the quantitative power of flow cytometry with the high content information associated with microscopy. The system can collect data on a wide range of applications including nuclear localization during a signal transduction cascade, measuring the apoptotic index and imaging of rare cell populations. New assay development also allows for the enumeration of protein aggregates and silicon oil droplets, a critical quality control assay for protein based pharmaceuticals and reagents.
演讲时长58 分钟
演讲语言:英语 English
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点此收看讲座


精彩内容分享
ImageStream
的应用:细胞死亡和自噬,细胞周期与有丝分裂,干细胞,微生物,寄生虫
http://www.ebiotrade.com/speech/down/Merck-Millipore/150519/ppt/10014.JPG

ImageStream
的应用:细胞信号转导,病原体内吞,细胞内共定位,细胞形变和趋化,细胞间相互作用
http://www.ebiotrade.com/speech/down/Merck-Millipore/150519/ppt/10013.JPG


Amnis
免疫检测方法
http://www.ebiotrade.com/speech/down/Merck-Millipore/150519/ppt/10027.JPG


免疫突触的形成
http://www.ebiotrade.com/speech/down/Merck-Millipore/150519/ppt/10018.JPG

制药配方中的颗粒分析
http://www.ebiotrade.com/speech/down/Merck-Millipore/150519/ppt/10031.JPG

Amnis
量化成像流式细胞仪用于颗粒分析 Vs. 其他平台
http://www.ebiotrade.com/speech/down/Merck-Millipore/150519/ppt/10045.JPG